Overview

  • Product name

  • Description

    Rabbit polyclonal to DTX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DTX1 aa 389-418 (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • HeLa cell line lysate; Human lymph tissue; Jurkat cells This antibody gave a positive result when used in the following methanol fixed cell lines: U-87 MG
  • General notes

    Previously labelled as Deltex.

Properties

Applications

Our Abpromise guarantee covers the use of ab135730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/100 - 1/500. Predicted molecular weight: 67 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.
  • Tissue specificity

    Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the Deltex family.
    Contains 1 RING-type zinc finger.
    Contains 2 WWE domains.
  • Domain

    The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.
  • Post-translational
    modifications

    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deltex 1, E3 ubiquitin ligase antibody
    • Deltex antibody
    • Deltex homolog 1 (Drosophila) antibody
    • Deltex homolog 1 antibody
    • Deltex protein 1 antibody
    • Deltex-1 antibody
    • Deltex1 antibody
    • dtx1 antibody
    • DTX1_HUMAN antibody
    • E3 ubiquitin protein ligase DTX1 antibody
    • E3 ubiquitin-protein ligase DTX1 antibody
    • FXI-T1 antibody
    • Fxit 1 antibody
    • Fxit1 antibody
    • hDTX1 antibody
    • hDx 1 antibody
    • mDTX1 antibody
    • Protein deltex 1 antibody
    • Protein deltex-1 antibody
    see all

Images

  • ab135730 stained U-87 MG cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135730 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DTX1 antibody (ab135730) at 1/100 dilution + HeLa cell line lysate at 35 µg

    Predicted band size: 67 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph tissue labelling DTX1 with ab135730 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of Jurkat cells labelling DTX1 with ab135730 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135730 has not yet been referenced specifically in any publications.

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