Key features and details
- Rabbit polyclonal to DTX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DTX2 antibody
See all DTX2 primary antibodies
DescriptionRabbit polyclonal to DTX2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: RT4 cell lysate. IHC-P: Human esophagus tissue. ICC/IF: A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 67 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionRegulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the Deltex family.
Contains 1 RING-type zinc finger.
Contains 2 WWE domains.
DomainThe WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
- Information by UniProt
- Deltex 2 antibody
- deltex homolog 2 (Drosophila) antibody
- deltex, Drosophila, homolog of, 2 antibody
Anti-DTX2 antibody (ab243494) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 67 kDa
Paraffin-embedded human esophagus tissue stained for DTX2 using ab243494 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for DTX2 (green) using ab243494 at 4 µg/ml in ICC/IF. Microtubules are shown in red.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243494 has not yet been referenced specifically in any publications.