Product nameAnti-DTX2 antibody [EPR10916]
See all DTX2 primary antibodies
DescriptionRabbit monoclonal [EPR10916] to DTX2
Tested applicationsSuitable for: Flow Cyt, IP, IHC-P, WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis
Synthetic peptide within Human DTX2 aa 500-600. The exact sequence is proprietary.
Database link: Q86UW9
- HepG2 and MDA-MB-435 cell lysates, Human fetal heart lysate, Human cervical carcinoma and thyroid gland carcinoma tissues, HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 67 kDa.|
FunctionRegulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the Deltex family.
Contains 1 RING-type zinc finger.
Contains 2 WWE domains.
DomainThe WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
- Information by UniProt
- Deltex 2 antibody
- deltex homolog 2 (Drosophila) antibody
- deltex, Drosophila, homolog of, 2 antibody
All lanes : Anti-DTX2 antibody [EPR10916] (ab170907) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.
Flow cytometry analysis of permeabilized HepG2 cells labeling DTX2 (red), using ab170907 at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green)
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate showing DTX2, using ab170907 at 1/10 dilution.
ab170907 has not yet been referenced specifically in any publications.