Anti-DTX2 antibody [EPR10916] (ab170907)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DTX2 antibody [EPR10916]
See all DTX2 primary antibodies -
DescriptionRabbit monoclonal [EPR10916] to DTX2
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, IP, IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis
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Immunogen
Synthetic peptide within Human DTX2 aa 500-600. The exact sequence is proprietary.
Database link: Q86UW9 -
Positive control
- HepG2 and MDA-MB-435 cell lysates, Human fetal heart lysate, Human cervical carcinoma and thyroid gland carcinoma tissues, HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10916
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
Target
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FunctionRegulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the Deltex family.
Contains 1 RING-type zinc finger.
Contains 2 WWE domains. -
DomainThe WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.
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Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
- Information by UniProt
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Database links
- Entrez Gene: 113878 Human
- Entrez Gene: 74198 Mouse
- Entrez Gene: 304591 Rat
- Entrez Gene: 379387 Xenopus laevis
- Omim: 613141 Human
- SwissProt: Q86UW9 Human
- SwissProt: Q8R3P2 Mouse
- Unigene: 187058 Human
see all -
Alternative names
- Deltex 2 antibody
- deltex homolog 2 (Drosophila) antibody
- deltex, Drosophila, homolog of, 2 antibody
see all
Images
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![Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)](https://www.abcam.com/ps/products/170/ab170907/Images/ab170907-172091-ab170907WB1.jpg)
Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)All lanes : Anti-DTX2 antibody [EPR10916] (ab170907) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)](https://www.abcam.com/ps/products/170/ab170907/Images/ab170907-172096-ab170907IHC21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)](https://www.abcam.com/ps/products/170/ab170907/Images/ab170907-172094-ab170907IHC11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.
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![Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)](https://www.abcam.com/ps/products/170/ab170907/Images/ab170907-172093-ab170907FC.jpg)
Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)Flow cytometry analysis of permeabilized HepG2 cells labeling DTX2 (red), using ab170907 at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green)
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![Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)](https://www.abcam.com/ps/products/170/ab170907/Images/ab170907-172092-ab170907IP.jpg)
Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate showing DTX2, using ab170907 at 1/10 dilution.
Protocols
Datasheets and documents
References
ab170907 has not yet been referenced specifically in any publications.
