RecombinantRabMAb

Recombinant Anti-DTX2 antibody [EPR10916] (ab170907)

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Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
  • Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)
  • Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)
  • Anti-DTX2 antibody [EPR10916] (ab170907)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10916] to DTX2
  • Suitable for: Flow Cyt, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DTX2 antibody [EPR10916]
    See all DTX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10916] to DTX2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DTX2 aa 500-600. The exact sequence is proprietary.
    Database link: Q86UW9

  • Positive control

    • HepG2 and MDA-MB-435 cell lysates, Human fetal heart lysate, Human cervical carcinoma and thyroid gland carcinoma tissues, HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10916

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
IP
Human
WB
Human
All applications
Mouse
Rat
Xenopus laevis
Application Abreviews Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the Deltex family.
      Contains 1 RING-type zinc finger.
      Contains 2 WWE domains.

    • Domain

      The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.

    • Cellular localization

      Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Deltex 2 antibody
      • deltex homolog 2 (Drosophila) antibody
      • deltex, Drosophila, homolog of, 2 antibody
      • Deltex2 antibody
      • DTX2 antibody
      • DTX2_HUMAN antibody
      • hDTX2 antibody
      • Highly similar to hPMSR6 [H.sapiens] antibody
      • KIAA1528 antibody
      • Probable E3 ubiquitin-protein ligase DTX2 antibody
      • Protein deltex 2 antibody
      • Protein deltex-2 antibody
      • RING finger protein 58 antibody
      • RNF58 antibody
      • Zinc ion binding protein antibody
      see all

    Images

    • Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)
      Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)
      All lanes : Anti-DTX2 antibody [EPR10916] (ab170907) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MDA-MB-435 cell lysate
      Lane 3 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 67 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)

      Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)
      Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)

      Flow cytometry analysis of permeabilized HepG2 cells labeling DTX2 (red), using ab170907 at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green)

    • Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)
      Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)

      Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate showing DTX2, using ab170907 at 1/10 dilution.

    • Anti-DTX2 antibody [EPR10916] (ab170907)
      Anti-DTX2 antibody [EPR10916] (ab170907)

    References (0)

    Publishing research using ab170907? Please let us know so that we can cite the reference in this datasheet.

    ab170907 has not yet been referenced specifically in any publications.

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