• Product name

  • Description

    Rabbit polyclonal to DTX3L
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 740) of human DTX3L (NP_612144.1)

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.



Our Abpromise guarantee covers the use of ab70621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the Deltex family.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B lymphoma and BAL associated protein antibody
    • B-lymphoma- and BAL-associated protein antibody
    • BBAP antibody
    • Deltex 3 like antibody
    • DTX 3L antibody
    • DTX3L antibody
    • DTX3L_HUMAN antibody
    • E3 ubiquitin-protein ligase DTX3L antibody
    • Protein deltex 3 like antibody
    • Protein deltex 3 like protein antibody
    • Protein deltex-3-like antibody
    • Rhysin 2 antibody
    • Rhysin-2 antibody
    • Rhysin2 antibody
    see all


  • All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 83 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.


ab70621 has not yet been referenced specifically in any publications.

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