Product nameAnti-DTX3L antibody
See all DTX3L primary antibodies
DescriptionRabbit polyclonal to DTX3L
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 740) of human DTX3L (NP_612144.1)
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionUbiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the Deltex family.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- B lymphoma and BAL associated protein antibody
- B-lymphoma- and BAL-associated protein antibody
- BBAP antibody
All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.
ab70621 has not yet been referenced specifically in any publications.