The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
Use at 2-5 µg/mg of lysate.
Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation.
Protein modification; protein ubiquitination.
Belongs to the Deltex family. Contains 1 RING-type zinc finger.
All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.