Product nameAnti-DTYMK antibody
See all DTYMK primary antibodies
DescriptionRabbit polyclonal to DTYMK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human DTYMK aa 162-212. The exact sequence is proprietary.
Database link: P23919
- WB: HeLa, HEK-293T, Jurkat, NIH/3T3 and TCMK-1 whole cell lysate. IP: DTYMK IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to DTYMK immobilized on solid support.
Our Abpromise guarantee covers the use of ab241493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 μl/mg lysate
FunctionCatalyzes the conversion of dTMP to dTDP.
PathwayPyrimidine metabolism; dTTP biosynthesis.
Sequence similaritiesBelongs to the thymidylate kinase family.
- Information by UniProt
- CDC8 antibody
- Deoxythymidylate kinase (thymidylate kinase) antibody
- deoxythymidylate kinase antibody
DTYMK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241493 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab241493 at 1/100 dilution.
Lane 1: ab241493 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-DTYMK antibody (ab241493) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
ab241493 has not yet been referenced specifically in any publications.