Dual color: FITC + PE Anti-CD3 + CD8 antibody [CA17.2A12 + YCATE 55.9] (ab34266)
![Flow Cytometry - Dual color: FITC + PE Anti-CD3 + CD8 antibody [CA17.2A12 + YCATE 55.9] (ab34266)](https://www.abcam.com/ps/products/34/ab34266/Images/ab34266-344228-anticd3cd8antibodyCA172A12YCATE-559-Dual-color-FITCPE-flowcytometry-blood-canine.jpg "Flow Cytometry - Dual color: FITC + PE Anti-CD3 + CD8 antibody [CA17.2A12 + YCATE 55.9] (ab34266)")
Key features and details
- Dual color: FITC + PE Mouse monoclonal [CA17.2A12 + YCATE 55.9] to CD3 + CD8
- Suitable for: Flow Cyt
- Reacts with: Dog
- Conjugation: Dual color: FITC + PE
- Isotype: IgG1
Overview
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Product name
Dual color: FITC + PE Anti-CD3 + CD8 antibody [CA17.2A12 + YCATE 55.9] -
Description
Dual color: FITC + PE Mouse monoclonal [CA17.2A12 + YCATE 55.9] to CD3 + CD8 -
Host species
Mouse -
Conjugation
Dual color: FITC + PE -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Dog -
Immunogen
This product was produced with the following immunogens:
Full length native protein (purified). affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90. (Clone CA17.2A12)
Fusion protein. CD8 alpha chimaeric human IgG1 Fc fusion protein. (Clone YCATE 55.9) -
General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail consists of: CD3 = Clone:CA17.2A12, Isotype:IgG1, Label:FITC. Raised in Mouse. CD8 = Clone:YCATE 55.9, Isotype:IgG1, Label:PE. Raised in Rat.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Please see notes section. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, Sucrose, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CA17.2A12 + YCATE 55.9 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab34266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use 10µl for 106 cells in 100µl. |
Target
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Relevance
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. -
Cellular localization
Membrane; single pass type I membrane protein. -
Alternative names
- CD3D antibody
- CD3d molecule antibody
- CD3E antibody
see all
Images
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Flow Cytometry - Dual color: FITC + PE Anti-CD3 + CD8 antibody [CA17.2A12 + YCATE 55.9] (ab34266)
Flow cytometry analysis of red cell lysed canine blood gated on mononuclear cells labeling CD3 + CD8 with ab34266.
Datasheets and documents
References (0)
ab34266 has not yet been referenced specifically in any publications.
