Dual color: FITC + PE Anti-CD4 + CD8 antibody [YKIX 302.9 + YCATE 55.9] (ab34267)
![Flow Cytometry - Dual color: FITC + PE Anti-CD4 + CD8 antibody [YKIX 302.9 + YCATE 55.9] (ab34267)](https://www.abcam.com/ps/products/34/ab34267/Images/ab34267-344229-antiCD4CD8antibodyYKIX-3029YCATE-559Dual-color-FITC--PE-flowcytometry-blood-dog.jpg "Flow Cytometry - Dual color: FITC + PE Anti-CD4 + CD8 antibody [YKIX 302.9 + YCATE 55.9] (ab34267)")
Key features and details
- Dual color: FITC + PE Rat monoclonal [YKIX 302.9 + YCATE 55.9] to CD4 + CD8
- Suitable for: Flow Cyt
- Reacts with: Dog
- Conjugation: Dual color: FITC + PE
Overview
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Product name
Dual color: FITC + PE Anti-CD4 + CD8 antibody [YKIX 302.9 + YCATE 55.9]
See all CD4 + CD8 primary antibodies -
Description
Dual color: FITC + PE Rat monoclonal [YKIX 302.9 + YCATE 55.9] to CD4 + CD8 -
Host species
Rat -
Conjugation
Dual color: FITC + PE -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Dog -
Immunogen
This product was produced with the following immunogens:
Tissue, cells or virus. Concanavallin A activated T cell blasts. (CloneYKIX302.9)
Fusion protein corresponding to Dog CD4 + CD8. CD8 alpha chimaeric human IgG1 Fc fusion protein. (Clone YCATE55.9)
Database link: P33705 -
General notes
This cocktail consists of: CD4 = Clone:YKIX 302.9, Isotype:IgG1, Label:FITC CD8 = Clone:YCATE 55.9, Isotype:IgG2a, Label:PEab34267 should be stored undiluted. It is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Please see notes section. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS, 5% Sucrose -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
YKIX 302.9 + YCATE 55.9 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. |
Target
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Relevance
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. -
Cellular localization
Membrane; single pass type I membrane protein. -
Alternative names
- CD4 antigen antibody
- CD4 molecule antibody
- CD4 mut antibody
see all
Images
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Flow Cytometry - Dual color: FITC + PE Anti-CD4 + CD8 antibody [YKIX 302.9 + YCATE 55.9] (ab34267)
Flow cytometry analysis of red cell lysed canine blood gated on mononuclear cells labeling CD4 + CD8 with ab34267.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab34267 has not yet been referenced specifically in any publications.