Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
![Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)](https://www.abcam.com/ps/products/1/ab1285/Images/ab1285-4144-mouse-igg1monoclonal-hybigg1-fitc-phycoerythrin-isotype-control-immunocytochemistry-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)")
Overview
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Product name
Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control
See all Mouse isotype controls -
Conjugation
Dual color: FITC + PE -
Tested applications
Suitable for: Flow Cytmore details -
General notes
This product is a mixture of the same clone separately bearing both FITC and PE labels. This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Isotype control notes
This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies. -
Clonality
Monoclonal -
Clone number
HybIgG1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells. |
Images
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Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)ICC/IF image of ab2185 stained MCF7 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References (0)
ab1285 has not yet been referenced specifically in any publications.
