Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)

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Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)

    Overview

    • Product name

      Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control
      See all Mouse isotype controls

    • Conjugation

      Dual color: FITC + PE

    • Tested applications

      Suitable for: Flow Cytmore details

    • General notes

      This product is a mixture of the same clone separately bearing both FITC and PE labels. This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.


      This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 0.5% BSA
    • Concentration information loading...

    • Isotype control notes

      This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

    • Clonality

      Monoclonal

    • Clone number

      HybIgG1

    • Myeloma

      unknown

    • Isotype

      IgG1

    • Light chain type

      unknown

    • Research areas

    • Alternative names

      • Mouse Isotype Control

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1285 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells.

    Images

    • Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
      Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
      ICC/IF image of ab2185 stained MCF7 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (0)

    Publishing research using ab1285? Please let us know so that we can cite the reference in this datasheet.

    ab1285 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Q&A
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