Key features and details
- Rabbit polyclonal to DUBA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DUBA antibody
See all DUBA primary antibodies
DescriptionRabbit polyclonal to DUBA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human DUBA aa 150-500. The exact sequence is proprietary. NP_060072.1
Database link: Q96G74
- HeLa, 293T and NIH3T3 cell lysates
Protein previously labeled as OTUD5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNegative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
Tissue specificityExpressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
Sequence similaritiesBelongs to the peptidase C85 family.
Contains 1 OTU domain.
- Information by UniProt
- Deubiquinating enzyme A antibody
- Deubiquitinating enzyme A antibody
- DKFZp761A052 antibody
Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate, using chemiluminescence with exposure times of 10 seconds.
All lanes : Anti-DUBA antibody (ab176727) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 61 kDa
Chemiluminescence with exposure times of 3 minutes.
ab176727 has been referenced in 1 publication.
- Cho JH et al. The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3. J Biol Chem 292:11804-11814 (2017). PubMed: 28559278