Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
Belongs to the peptidase C85 family. Contains 1 OTU domain.
Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate, using chemiluminescence with exposure times of 10 seconds.
Western blot - Anti-DUBA antibody (ab176727)
All lanes : Anti-DUBA antibody (ab176727) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 61 kDa
Chemiluminescence with exposure times of 3 minutes.