Product nameAnti-DUBA antibody
See all DUBA primary antibodies
DescriptionRabbit polyclonal to DUBA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human DUBA aa 150-500. The exact sequence is proprietary. NP_060072.1
Database link: Q96G74
- HeLa, 293T and NIH3T3 cell lysates
Protein previously labeled as OTUD5.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNegative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
Tissue specificityExpressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
Sequence similaritiesBelongs to the peptidase C85 family.
Contains 1 OTU domain.
- Information by UniProt
- Deubiquinating enzyme A antibody
- Deubiquitinating enzyme A antibody
- DKFZp761A052 antibody
Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate, using chemiluminescence with exposure times of 10 seconds.
All lanes : Anti-DUBA antibody (ab176727) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 61 kDa
Chemiluminescence with exposure times of 3 minutes.
This product has been referenced in:
- Cho JH et al. The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3. J Biol Chem 292:11804-11814 (2017). Read more (PubMed: 28559278) »