Key features and details
- Rabbit polyclonal to DUBA
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DUBA antibody
See all DUBA primary antibodies
DescriptionRabbit polyclonal to DUBA
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human DUBA aa 521-571. The exact sequence is proprietary. (NP_060072.1).
Database link: Q96G74
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Protein previously labeled as OTUD5.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225558 was affinity purified using an epitope specific to DUBA immobilized on solid support.
Our Abpromise guarantee covers the use of ab225558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
FunctionNegative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
Tissue specificityExpressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
Sequence similaritiesBelongs to the peptidase C85 family.
Contains 1 OTU domain.
- Information by UniProt
- Deubiquinating enzyme A antibody
- Deubiquitinating enzyme A antibody
- DKFZp761A052 antibody
All lanes : Anti-DUBA antibody (ab225558) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds
DUBA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225558 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225558 at 1 µg/ml.
Lane 1: ab225558 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225558 has not yet been referenced specifically in any publications.