ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis.
Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
In the N-terminal section; belongs to the peroxidase family. Contains 3 EF-hand domains. Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain.
Widely expressed in fetal tissues.
Apical cell membrane. Localizes to the apical membrane of epithelial cells.
Anti-DUOX2 antibody (ab170308) at 1/100 dilution + CEM cell lysate at 35 µg
Predicted band size: 175 kDa
Flow Cytometry - Anti-DUOX2 antibody (ab170308)
Flow cytometric analysis of CEM cells labeling DUOX2 with ab170308 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.