Overview

  • Product name
  • Description
    Rabbit polyclonal to DUOX2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DUOX2 aa 520-550 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NRD8

  • Positive control
    • CEM cell lysate; CEM cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170308 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 175 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
  • Tissue specificity
    Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis.
  • Pathway
    Hormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in disease
    Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similarities
    In the N-terminal section; belongs to the peroxidase family.
    Contains 3 EF-hand domains.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Developmental stage
    Widely expressed in fetal tissues.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Apical cell membrane. Localizes to the apical membrane of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual oxidase 2 antibody
    • Dual oxidase like domains 2 antibody
    • Dual oxidase2 antibody
    • DUOX2 antibody
    • DUOX2_HUMAN antibody
    • Flavoprotein NADPH oxidase antibody
    • Large NOX 2 antibody
    • LNOX2 antibody
    • Long NOX 2 antibody
    • NADH/NADPH thyroid oxidase p138 tox antibody
    • NADH/NADPH thyroid oxidase p138-tox antibody
    • NADPH oxidase/peroxidase DUOX2 antibody
    • NADPH thyroid oxidase 2 antibody
    • Nicotinamide adenine dinucleotide phosphate oxidase antibody
    • NOXEF2 antibody
    • p138 thyroid oxidase antibody
    • P138 TOX antibody
    • TDH6 antibody
    • THOX2 antibody
    • Thyroid oxidase 2 antibody
    see all

Images

  • Anti-DUOX2 antibody (ab170308) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size: 175 kDa

  • Flow cytometric analysis of CEM cells labeling DUOX2 with ab170308 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.

References

ab170308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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