Key features and details
- Rabbit polyclonal to DUOX2
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-DUOX2 antibody
DescriptionRabbit polyclonal to DUOX2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Human, Chimpanzee, Macaque monkey, Orangutan
Synthetic peptide corresponding to Rat DUOX2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: Q9ES45
- This antibody gave a positive signal in Rat Colon tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Rat Thyroid.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 175 kDa.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGenerates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
Tissue specificityExpressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis.
PathwayHormone biosynthesis; thyroid hormone biosynthesis.
Involvement in diseaseDefects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
Sequence similaritiesIn the N-terminal section; belongs to the peroxidase family.
Contains 3 EF-hand domains.
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
Developmental stageWidely expressed in fetal tissues.
Cellular localizationApical cell membrane. Localizes to the apical membrane of epithelial cells.
- Information by UniProt
- Dual oxidase 2 antibody
- Dual oxidase like domains 2 antibody
- Dual oxidase2 antibody
IHC image of DUOX2 staining in Rat thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97266, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-DUOX2 antibody (ab97266) at 1 µg/ml (Milk blocking (3%)) + Colon (Rat) Tissue Lysate at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
ab97266 has been referenced in 1 publication.
- Dho SH et al. NOX5-L can stimulate proliferation and apoptosis depending on its levels and cellular context, determining cancer cell susceptibility to cisplatin. Oncotarget 6:39235-46 (2015). PubMed: 26513170