Product nameAnti-DUS2L antibody - C-terminal
See all DUS2L primary antibodies
DescriptionRabbit polyclonal to DUS2L - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DUS2L aa 443-493 (C terminal). The exact sequence is proprietary.
Database link: Q9NX74
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226252 was affinity purified using an epitope specific to DUS2L immobilized on solid support.
Our Abpromise guarantee covers the use of ab226252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceDUS2L is a dihydrouridine synthase that catalyzes the reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA.
Cellular localizationCytoplasmic and Endoplasmic reticulum. Note=Mainly at the endoplasmic reticulum.
- 2310016K04Rik antibody
- dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae) antibody
- DUS2 antibody
All lanes : Anti-DUS2L antibody - C-terminal (ab226252) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
DUS2L was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226252 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226252 at 0.4 µg/ml.
Lane 1: ab226252 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226252 has not yet been referenced specifically in any publications.