Overview

  • Product name

    Anti-DUS2L antibody [EPR14042]
    See all DUS2L primary antibodies
  • Description

    Rabbit monoclonal [EPR14042] to DUS2L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DUS2L aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NX74

  • Positive control

    • HeLa, A549 and A431 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/40 - 1/60.
ICC/IF 1/100.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    DUS2L is a dihydrouridine synthase that catalyzes the reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA.
  • Cellular localization

    Cytoplasmic and Endoplasmic reticulum. Note=Mainly at the endoplasmic reticulum.
  • Database links

  • Alternative names

    • 2310016K04Rik antibody
    • dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae) antibody
    • DUS2 antibody
    • FLJ20399 antibody
    • SMM1 antibody
    • tRNA dihydrouridine synthase 2 like antibody
    • Up regulated in lung cancer antibody
    • URLC8 antibody
    see all

Images

  • All lanes : Anti-DUS2L antibody [EPR14042] (ab181262) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 55 kDa

  • Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab181262 has not yet been referenced specifically in any publications.

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