Anti-DUSP10 antibody (ab228987)
Key features and details
- Rabbit polyclonal to DUSP10
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DUSP10 antibody -
Description
Rabbit polyclonal to DUSP10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human DUSP10 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y6W6 -
Positive control
- IHC-P: U87 xenograft tissue. WB: HeLa and HCT 116 whole cell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 541175 Cow
- Entrez Gene: 11221 Human
- Entrez Gene: 63953 Mouse
- Entrez Gene: 63995 Rat
- Entrez Gene: 705056 Rhesus monkey
- Omim: 608867 Human
- SwissProt: Q0IID7 Cow
- SwissProt: Q9Y6W6 Human
see all -
Alternative names
- Dual specificity phosphatase 10 antibody
- Dual specificity phosphatase MKP 5 antibody
- Dual specificity phosphatase MKP5 antibody
see all
Images
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All lanes : Anti-DUSP10 antibody (ab228987) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa10% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DUSP10 (green) using ab228987 (1/200 dilution) in ICC/IF. Cells were fixed in paraformaldehyde. Counterstain: Hoechst 33342 (blue).
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Paraffin-embedded U87 xenograft tissue stained for DUSP10 with ab228987 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228987 has not yet been referenced specifically in any publications.