Product nameAnti-DUSP14 antibody
See all DUSP14 primary antibodies
DescriptionRabbit polyclonal to DUSP14
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- WB: HEK-293T cells. IHC-P: Human small intestine tissue. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the inactivation of MAP kinases. Dephosphorylates ERK, JNK and p38 MAP-kinases.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.
- Information by UniProt
- Dual specificity phosphatase 14 antibody
- Dual specificity protein phosphatase 14 antibody
- DUS14_HUMAN antibody
Lanes 2-3 : Anti-DUSP14 antibody (ab272587) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Control (vector only transfected HEK-293T lysate)
Lane 3 : DUSP14 over-expression lysate (mammalian HEK-293T cells)
Paraffin-embedded formalin-fixed Human small intestine tissue stained for DUSP14 using ab272587 at 1/200 in immunohistochemical analysis.
PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling DUSP14 (green) using ab272587 at 2 ug/ml in ICC/IF analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272587 has not yet been referenced specifically in any publications.