Overview

  • Product name
    Anti-DUSP16 antibody - C-terminal
    See all DUSP16 primary antibodies
  • Description
    Rabbit polyclonal to DUSP16 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 461-665 of Human DUSP16 (UniProt Q9BY84).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cell lysates; A431 cells; HT-29 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 16 antibody
    • Dual specificity protein phosphatase 16 antibody
    • DUS16_HUMAN antibody
    • DUSP 16 antibody
    • DUSP16 antibody
    • KIAA1700 antibody
    • MAP kinase phosphatase 7 antibody
    • MAPK phosphatase 7 antibody
    • Mitogen activated protein kinase phosphatase 7 antibody
    • Mitogen-activated protein kinase phosphatase 7 antibody
    • MKP 7 antibody
    • MKP-7 antibody
    • MKP7 antibody
    see all

Images

  • Anti-DUSP16 antibody - C-terminal (ab155139) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling DUSP16 with ab155139 at 1/500 dilution. Alpha-tubulin filaments were labeled red.
  • Immunohistochemical analysis of paraffin-embedded HT-29 xenograft labeling DUSP16 with ab155139 at 1/100 dilution.

References

ab155139 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155139.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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