Overview

  • Product name
  • Description
    Mouse polyclonal to DUSP18
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-188 of Human DUSP18 (NP_689724.3).

  • Positive control
    • DUSP18 transfected 293T cell lysate; HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab168173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).
  • Tissue specificity
    Widely expressed with highest levels in liver, brain, ovary and testis.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity protein phosphatase 18 antibody
    • DUS18_HUMAN antibody
    • Dusp18 antibody
    • LMW-DSP20 antibody
    • LMWDSP20 antibody
    • Low molecular weight dual specificity phosphatase 20 antibody
    see all

Images

  • All lanes : Anti-DUSP18 antibody (ab168173) at 1 µg/ml

    Lane 1 : DUSP18 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 21 kDa

  • Immunofluorescent analysis of HepG2 cells labeling DUSP18 with ab168173 at 10 µg/ml.

References

ab168173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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