Key features and details
- Rabbit polyclonal to DUSP22
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DUSP22 antibody
See all DUSP22 primary antibodies
DescriptionRabbit polyclonal to DUSP22
Specificityab70124 detects endogenous levels of total DUSP22 protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from an internal sequence of human DUSP22.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab70124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
FunctionActivates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).
Tissue specificityUbiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Dual specificity protein phosphatase 22 antibody
- DUS22_HUMAN antibody
- Dusp22 antibody
All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : RAW264.7 cell extracts
Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab70124 has been referenced in 1 publication.
- Feng Z et al. Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression. Theranostics 9:3501-3514 (2019). PubMed: 31281493