Anti-DUSP26 antibody (ab224407)
Key features and details
- Rabbit polyclonal to DUSP26
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DUSP26 antibody -
Description
Rabbit polyclonal to DUSP26 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human DUSP26 aa 1-100.
Database link: Q9BV47 -
Positive control
- IHC-P: Human prostate and cerebellum tissue. WB: DUSP26 overexpression HEK-293T lysate. ICC/IF: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Inactivates MAPK1 and MAPK3 which leads to dephosphorylation of heat shock factor protein 4 and a reduction in its DNA-binding activity. Inhibits MAP kinase p38 by dephosphorylating it and inhibits p38-mediated apoptosis in anaplastic thyroid cancer cells. Can also induce activation of MAP kinase p38 and c-Jun N-terminal kinase (JNK). -
Tissue specificity
Brain. In the brain it is expressed ubiquitously except in the hippocampus. Expressed in embryonal cancers (retinoblastoma, neuroepithilioma and neuroblastoma) and in anaplatic thyroid cancer. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. Nucleus. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 533896 Cow
- Entrez Gene: 78986 Human
- Entrez Gene: 66959 Mouse
- Entrez Gene: 100173535 Orangutan
- Entrez Gene: 306527 Rat
- SwissProt: Q17QJ3 Cow
- SwissProt: Q9BV47 Human
- SwissProt: Q9D700 Mouse
see all -
Alternative names
- DSP-4 antibody
- Dual specificity phosphatase 26 (putative) antibody
- Dual specificity phosphatase 26 antibody
see all
Images
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Paraffin-embedded human cerebellum tissue stained for DUSP26 using ab224407 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-DUSP26 antibody (ab224407) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : DUSP26 overexpression HEK-293T (co-expressed with a C terminal myc-DDK tag, ~3.1 kDa) lysate
Predicted band size: 24 kDa -
Paraffin-embedded human prostate tissue stained for DUSP26 using ab224407 at 1/50 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for DUSP26 (green) using ab224407 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224407 has been referenced in 1 publication.
- Chen J et al. Decreased DUSP26 Expression Promotes Malignant Behavior in Glioblastoma Cells via Deregulation of MAPK and Akt Signaling Pathway. Front Oncol 11:622826 (2021). PubMed: 33718185