Overview

  • Product name

  • Description

    Rabbit polyclonal to DUSP27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 250-282 (EALMTVRKKR AIYPNEGFLK QLRELNEKLM EER) of Human DUSP27 (Q5VZP5).

  • Positive control

    • Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab121372 at 1/100 dilution staining DUSP27 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

References

ab121372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (mouse muscle C2C12)
Specification
mouse muscle C2C12
Treatment
siRNA transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 09 2013

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Cell lysate - whole cell (Kidney)
Specification
Kidney
Treatment
plasmid transfected
Total protein in input
500 µg

Abcam user community

Verified customer

Submitted Sep 09 2013

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Sample
Mouse Cell (mouse muscle C2C12)
Specification
mouse muscle C2C12
Permeabilization
Yes - 0.3% triton in PBS
Fixative
Paraformaldehyde

Yanfeng Valued Customer

Verified customer

Submitted Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up