Overview

  • Product name

  • Description

    Rabbit polyclonal to DUSP2/PAC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DUSP2/PAC1 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: NP_004409

  • Positive control

    • WB: Mouse liver tissue lysates. Flow: HepG2 cells.
  • General notes

     This product was previously labelled as DUSP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab95159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 34 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
  • Tissue specificity

    Expressed in hematopoietic tissues.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity phosphatase 2 antibody
    • Dual specificity protein phosphatase 2 antibody
    • Dual specificity protein phosphatase PAC 1 antibody
    • Dual specificity protein phosphatase PAC-1 antibody
    • DUS2_HUMAN antibody
    • DUSP 2 antibody
    • DUSP2 antibody
    • PAC 1 antibody
    • PAC1 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

Images

  • Anti-DUSP2/PAC1 antibody (ab95159) at 1/50 dilution + Mouse liver tissue lysates at 35 µg

    Predicted band size: 34 kDa

  • Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DUSP2/PAC1 (Right) compared to a negative control cell (Left) using ab95159 at 1/10 dilution followed by a FITC-conjugated goat-anti-rabbit secondary antibody.

References

ab95159 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

I just wanted to check in with you regarding the issues that you had been experiencing the DUSP7 and DUSP2 antibodies.

Have you had an opportunity to assemble information about your protocol? We take issues such as these seriously and we guarantee our products for a period of 6 months if used the manner specified on our datasheets. We ask for protocol details to determine if the product is being used consistent with the Abpromise.

It is also very important that we have the Abcam confirmation number or the PO number used to purchase the product as I would need these to issue a replacement or refund.

Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for your response.

I am sorry about the issues you have been having. Thank you for bringing this to our attention here and with Jason. Unfortunately the image did not arrive with this. It may be that the file size is too large for our email system.

As company policy, in order to send a replacement we do need the particulars of your protocol. This information is important not only in helping us understand the problems that you are experiencing but in our internal testing as well.

I see that you have been working with Mouse ESCs, could you tell me the lysis buffer that you have used?
Have you reduced and denatured your samples? Which method/buffers did you use?
How much total protein did you use per well?
Did you load a loading control? Which lc did you use? What were the results?
Did you load a positive control? Which pc did you use? What were the results?
What membrane type did you transfer to?
What blocking steps did you use?
What dilutions of this product did you use? How long and at what temperature was this antibody incubated?
What was the secondary antibody that you have used?How long and at what temperature was this antibody incubated?
Have you run a no primary antibody control blot? What were the results?
What is the wash buffer that you use?
Could you provide the Abcam order confirmation number or PO number used to purchase this?

Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.

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Answer

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Often times high background can be reduced by using TBS + 0.1% Tween 20 for your washes or by reducing the concentration of your antibodies. Would you mind sending me the details of your protocol including the tissue type you are working with, your sample preparations including denaturing and reducing steps, the amount of total protein per well, the positive controls you have used, loading controls or poncheau staining results, blocking procedures, antibody incubation steps, washes, images and any steps that you may have changed while troubleshooting.

These details will not only help me to better understand your difficulties and be able to offer suggestions but will also be used for internal testing of the products if deemed neccessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee.


Please let me know if you have any questions.

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Answer

DISCOUNT CODE: XXXXXXX Expiration date: XXXXXXX I am very pleased to hear you would like to accept our offer and test ab95159 in rat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Answer

Thank you for your enquiry and your interest. The peptide antigen of ab95159 shares 100% homology with rat DUSP2.  We have not tested its cross-reactivity with rat.  Please let me know if you have any additional questions. 

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