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I am writing about Abs questions from Abcam.
Abcam provides good antibodies, and we are happy to order products from Abcam. We ordered Dusp2 (ab95159) and Dusp7 (ab100921) from Abcam, and used them for WB in mouse embryonic stem cells. But we found the antibody is not good as showed in the websites. There were a lot of unspecific bands (we can send you images later), and the predicted bands are very weak compared to other unspecific bands.
So I wonder if we can replace them or get the money back?
Asked on Jan 23 2012
Thank you for contacting Abcam.
I am sorry to hear of the difficulties that you have been experiencing using these products. Often times high background can be reduced by using TBS + 0.1% Tween 20 for your washes or by reducing the concentration of your antibodies. Would you mind sending me the details of your protocol including the tissue type you are working with, your sample preparations including denaturing and reducing steps, the amount of total protein per well, the positive controls you have used, loading controls or poncheau staining results, blocking procedures, antibody incubation steps, washes, images and any steps that you may have changed while troubleshooting.
These details will not only help me to better understand your difficulties and be able to offer suggestions but will also be used for internal testing of the products if deemed neccessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee.
Please let me know if you have any questions.
Answered on Jan 23 2012