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Hi Abcam, We have tried many ways, like different Ab dilution, wash times, sample preparation et al. The result is not good, as you can see the Attached typical gel image. I also showed the images when Abcam had a show in UGA this week. Can you send us an alternative Dusp2/7 antibodies ?
Asked on Feb 17 2012
Thank you for your response.
I am sorry about the issues you have been having. Thank you for bringing this to our attention here and with Jason. Unfortunately the image did not arrive with this. It may be that the file size is too large for our email system.
As company policy, in order to send a replacement we do need the particulars of your protocol. This information is important not only in helping us understand the problems that you are experiencing but in our internal testing as well.
I see that you have been working with Mouse ESCs, could you tell me the lysis buffer that you have used?
Have you reduced and denatured your samples? Which method/buffers did you use?
How much total protein did you use per well?
Did you load a loading control? Which lc did you use? What were the results?
Did you load a positive control? Which pc did you use? What were the results?
What membrane type did you transfer to?
What blocking steps did you use?
What dilutions of this product did you use? How long and at what temperature was this antibody incubated?
What was the secondary antibody that you have used?How long and at what temperature was this antibody incubated?
Have you run a no primary antibody control blot? What were the results?
What is the wash buffer that you use?
Could you provide the Abcam order confirmation number or PO number used to purchase this?
Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.
Answered on Feb 17 2012