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    dusp3-antibody-epr5492-ab125077.pdf

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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-DUSP3 antibody [EPR5492] (ab125077)

  • Datasheet
  • SDS
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Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
  • Anti-DUSP3 antibody [EPR5492] (ab125077)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5492] to DUSP3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-DUSP3 antibody [EPR5492]
    See all DUSP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5492] to DUSP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DUSP3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, MCF7 and K562 cell lysates; Human ovarian cancer tissue and Mouse kidney tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5492
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • K562 nuclear extract lysate (ab14851)
    • MCF7 nuclear extract lysate (ab14860)

Applications

Our Abpromise guarantee covers the use of ab125077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P51452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1845 Human
    • Entrez Gene: 72349 Mouse
    • Entrez Gene: 498003 Rat
    • GenBank: NP_004081.1 Human
    • Omim: 600183 Human
    • SwissProt: P51452 Human
    • SwissProt: Q9D7X3 Mouse
    • Unigene: 181046 Human
    • Unigene: 196295 Mouse
    • Unigene: 8286 Rat
    see all
  • Alternative names

    • Dual specificity phosphatase 3 antibody
    • Dual specificity protein phosphatase 3 antibody
    • Dual specificity protein phosphatase VHR antibody
    • DUS3_HUMAN antibody
    • DUSP3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Vaccinia H1-related phosphatase antibody
    • Vaccinia virus phosphatase VH1 related antibody
    • VHR antibody
    see all

Images

  • Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Western blot - Anti-DUSP3 antibody [EPR5492] (ab125077)
    All lanes : Anti-DUSP3 antibody [EPR5492] (ab125077) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)

    ab125077, at 1/100, staining DUSP3 in paraffin-embedded Human ovarian cancer tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] (ab125077)

    ab125077, at 1/100, staining DUSP3 in paraffin-embedded Mouse kidney tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-DUSP3 antibody [EPR5492] (ab125077)
    Anti-DUSP3 antibody [EPR5492] (ab125077)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab125077? Please let us know so that we can cite the reference in this datasheet.

    ab125077 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Product code: 125077
    Inquiry: Hi, Could you let me know where in the Dusp3 protein the Anti-DUSP3 antibody [EPR5492] binds to please? I don't need to know the exact sequence but I want to be sure it is downstream of my predicted knockout target exon Many thanks,

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2012

    Answer

    Thank you for contacting us and your interest in our products.

    The peptide used to raise the anti-DUSP3 antibody [EPR5492] (ab125077) has been taken from the region ofamino acids 1 - 30 of theN-terminus of human DUSP3/VHR (UniProt: P51452).


    I hope this information has been sufficient to answer your question. If you have any further questions please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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