Overview

  • Product name
    Anti-DUSP4 antibody [EPR19881]
    See all DUSP4 primary antibodies
  • Description
    Rabbit monoclonal [EPR19881] to DUSP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human DUSP4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13115

  • Positive control
    • WB: MDA-MB-231, A549, SK-BR-3, HCT 116, RAW 264.7, PC-12 and C6 whole cell lysates; Human breast cancer lysate. ICC/IF: A549 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells. IP: MDA-MB-231 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 4 antibody
    • Dual specificity protein phosphatase 4 antibody
    • Dual specificity protein phosphatase hVH2 antibody
    • DUS4_HUMAN antibody
    • DUSP 4 antibody
    • Dusp4 antibody
    • HVH 2 antibody
    • HVH2 antibody
    • MAP kinase phosphatase 2 antibody
    • Mitogen activated protein kinase phosphatase 2 antibody
    • Mitogen-activated protein kinase phosphatase 2 antibody
    • MKP 2 antibody
    • MKP-2 antibody
    • MKP2 antibody
    • Serine/threonine specific protein phosphatase antibody
    • TYP antibody
    • Typ1 antibody
    • VH 2 antibody
    • VH1 homologous phosphatase 2 antibody
    • VH2 antibody
    see all

Images

  • All lanes : Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
    Lane 3 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2:3 minutes; Lane 3: 30 seconds; Lane 4: 1 second. 

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on A549 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution + Human breast cancer lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1:3 minutes; Lane 2/3: 15 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on MDA-MB-231 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • DUSP4 was immunoprecipitated from 0.35mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab216576 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab216576 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

    Lane 1: MDA-MB-231 whole cell lysate, 10µg (Input).

    Lane 2: ab216576 IP in MDA-MB-231 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216576 in MDA-MB-231 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

This product has been referenced in:
  • Ding Y  et al. Novel clinicopathological and molecular characterization of metanephric adenoma: a study of 28 cases. Diagn Pathol 13:54 (2018). Read more (PubMed: 30111351) »
  • Edwards NA  et al. Knockdown of p66Shc Alters Lineage-Associated Transcription Factor Expression in Mouse Blastocysts. Stem Cells Dev 27:1479-1493 (2018). Read more (PubMed: 30091687) »
See all 2 Publications for this product

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