Recombinant
RabMAb

Recombinant Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

Overview

  • Product name

    Anti-DUSP4 antibody [EPR19881] - BSA and Azide free
    See all DUSP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19881] to DUSP4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13115

  • Positive control

    • WB: MDA-MB-231, A549, SK-BR-3, HCT 116, RAW 264.7, PC-12 and C6 whole cell lysates; Human breast cancer lysate. ICC/IF: A549 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells. IP: MDA-MB-231 whole cell lysate.
  • General notes

    Ab222487 is the carrier-free version of ab216576. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222487 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity phosphatase 4 antibody
    • Dual specificity protein phosphatase 4 antibody
    • Dual specificity protein phosphatase hVH2 antibody
    • DUS4_HUMAN antibody
    • DUSP 4 antibody
    • Dusp4 antibody
    • HVH 2 antibody
    • HVH2 antibody
    • MAP kinase phosphatase 2 antibody
    • Mitogen activated protein kinase phosphatase 2 antibody
    • Mitogen-activated protein kinase phosphatase 2 antibody
    • MKP 2 antibody
    • MKP-2 antibody
    • MKP2 antibody
    • Serine/threonine specific protein phosphatase antibody
    • TYP antibody
    • Typ1 antibody
    • VH 2 antibody
    • VH1 homologous phosphatase 2 antibody
    • VH2 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on A549 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on MDA-MB-231 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • DUSP4 was immunoprecipitated from 0.35mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab216576 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab216576 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate, 10µg (Input).

    Lane 2: ab216576 IP in MDA-MB-231 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216576 in MDA-MB-231 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

References

ab222487 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222487.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up