Recombinant Anti-DUSP5 antibody [EPR19684] (ab200708)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19684] to DUSP5
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-DUSP5 antibody [EPR19684]
See all DUSP5 primary antibodies -
Description
Rabbit monoclonal [EPR19684] to DUSP5 -
Host species
Rabbit -
Specificity
Due to low expression levels, treatment with growth factors may be necessary to induce the expression of DUSP5 in certain cell lines. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Non human primates -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate from MEFs serum starved and then treated for 2h with 20% FCS. ICC/IF: Wild-type MEF cells serum starved and then treated for 2h with 20% FCS.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19684 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
Please note, this antibody worked well in ICC/IF on 100% methanol fixed cells but not on 4% PFA fixed cells. |
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WB | (1) |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Milk recommended as blocking agent. |
Notes |
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ICC/IF
1/100. Please note, this antibody worked well in ICC/IF on 100% methanol fixed cells but not on 4% PFA fixed cells. |
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Milk recommended as blocking agent. |
Target
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Function
Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1847 Human
- Entrez Gene: 240672 Mouse
- Omim: 603069 Human
- SwissProt: Q16690 Human
- Unigene: 2128 Human
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Alternative names
- Dual specificity phosphatase 5 antibody
- Dual specificity protein phosphatase 5 antibody
- Dual specificity protein phosphatase hVH 3 antibody
see all
Images
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All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution
Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
MEF Wt and DUSP5 KO whole cell lysates used on this blot are from MEFs serum starved and then treated for 2h with 20% FCS.
These lysates were kindly provided by Steve Keyes from Dundee University.
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Ab200708 staining DUSP5 in wild-type MEF cells serum starved and then treated for 2h with 20% FCS (top panel) and DUSP5 knockout MEF cells serum starved and then treated for 2h with 20% FCS (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab200708 at 1/100 dilution and anti-alpha Tubulin antibody ab7291 at 1/1000 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml. Nuclear DNA was labelled in blue with DAPI.
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All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution
Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Lane 2 : Untreated MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with 100 ng/ml PMA for 48 hours whole cell lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutes -
Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/5000 dilution + Human DUSP5 full length recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Exposure time: 8 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant human full length protein was made in-house.
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Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution + Mouse DUSP5 full length recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Mouse DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant mouse full length protein was made in-house.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab200708 has been referenced in 20 publications.
- Zhang BN et al. Norepinephrine as the Intrinsic Contributor to Contact Lens-Induced Pseudomonas aeruginosa Keratitis. Invest Ophthalmol Vis Sci 64:26 (2023). WB ; Human . PubMed: 37234000
- Hua C et al. Human Papillomavirus Type 16 Early Protein E7 Activates Autophagy through Inhibition of Dual-Specificity Phosphatase 5. Oxid Med Cell Longev 2022:1863098 (2022). PubMed: 35368866
- Yang Z et al. BAF53A drives colorectal cancer development by regulating DUSP5-mediated ERK phosphorylation. Cell Death Dis 13:1049 (2022). WB ; Human . PubMed: 36526622
- Kanda Y et al. Down-regulation of dual-specificity phosphatase 6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2. Genes Cells 26:109-116 (2021). PubMed: 33249692
- Tan L et al. Thyroid hormone plus dual-specificity phosphatase-5 siRNA increases the number of cardiac muscle cells and improves left ventricular contractile function in chronic doxorubicin-injured hearts. Theranostics 11:4790-4808 (2021). PubMed: 33754028