Overview

  • Product name

    Anti-DUSP5 antibody [EPR19684]
    See all DUSP5 primary antibodies
  • Description

    Rabbit monoclonal [EPR19684] to DUSP5
  • Host species

    Rabbit
  • Specificity

    Due to low expression levels, treatment with growth factors may be necessary to induce the expression of DUSP5 in certain cell lines.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human DUSP5 aa 1 to the C-terminus.
    Database link: Q16690

  • Positive control

    • WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate from MEFs serum starved and then treated for 2h with 20% FCS. ICC/IF: Wild-type MEF cells serum starved and then treated for 2h with 20% FCS.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19684
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Please note, this antibody worked well in ICC/IF on 100% methanol fixed cells but not on 4% PFA fixed cells.

WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Milk recommended as blocking agent.

Target

  • Function

    Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity phosphatase 5 antibody
    • Dual specificity protein phosphatase 5 antibody
    • Dual specificity protein phosphatase hVH 3 antibody
    • Dual specificity protein phosphatase hVH3 antibody
    • DUS5_HUMAN antibody
    • DUSP 5 antibody
    • DUSP antibody
    • DUSP5 antibody
    • HVH 3 antibody
    • HVH3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH 3 antibody
    • VH1 like phosphatase 3 antibody
    • VH3 antibody
    see all

Images

  • All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution

    Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
    Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    MEF Wt and DUSP5 KO whole cell lysates used on this blot are from MEFs serum starved and then treated for 2h with 20% FCS.

    These lysates were kindly provided by Steve Keyes from Dundee University.

  • Ab200708 staining DUSP5 in wild-type MEF cells serum starved and then treated for 2h with 20% FCS (top panel) and DUSP5 knockout MEF cells serum starved and then treated for 2h with 20% FCS (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab200708 at 1/100 dilution and anti-alpha Tubulin antibody ab7291 at 1/1000 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml. Nuclear DNA was labelled in blue with DAPI.

  • All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution

    Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 2 : Untreated MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with 100 ng/ml PMA for 48 hours whole cell lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes
  • Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/5000 dilution + Human DUSP5 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant human full length protein was made in-house.

  • Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution + Mouse DUSP5 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 4 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant mouse full length protein was made in-house.

References

This product has been referenced in:

  • Du M  et al. microRNA-95 knockdown inhibits epithelial-mesenchymal transition and cancer stem cell phenotype in gastric cancer cells through MAPK pathway by upregulating DUSP5. J Cell Physiol N/A:N/A (2019). WB . Read more (PubMed: 31309567) »
  • Schmidt C  et al. Immunoproteasome Inhibition Impairs T and B Cell Activation by Restraining ERK Signaling and Proteostasis. Front Immunol 9:2386 (2018). WB ; Mouse . Read more (PubMed: 30416500) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Treatment
20% Serum for 120 min
Specification
Mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Apr 15 2016

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