Western blot abreview for Anti-DUSP6 antibody [EPR129Y]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary MEF)
Loading amount
10 µg
Specification
Primary MEF
Treatment
100 ng/µl TPA for 2 hours
Gel Running Conditions
Reduced Denaturing (4-14% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/4000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Positive control
Wild type primary murine embryonic fibroblasts (MEFs) untreated or treated with 100ngµl-1, 12-O-tetradecanoylphorbol-13-acetate (TPA) for 2 hours.
Negative control
MKP-3 null primary murine embryonic fibroblasts (MEFs) untreated or treated with 100ngµl-1, 12-O-tetradecanoylphorbol-13-acetate (TPA) for 2 hours.

Additional data

Additional Notes
This is a very specific antibody suitable for both western blotting and IHC. We have confirmed by comparison of wildtype and MKP-3 (DUSP6) null mouse cells and tissues. We have not tested it against MKP-3 from other species however due to the high sequence identity between MKPs it is very likely to work.

Graeme Stewart

Verified customer

Submitted Jul 24 2012

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