Overview

  • Product name

  • Description

    Rabbit polyclonal to DUSP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length Human DUSP7 (NM_001947).

  • Positive control

    • HepG2 cell lysate and Human fetal liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab100921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors.
  • Tissue specificity

    Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity phosphatase 7 antibody
    • Dual specificity protein phosphatase 7 antibody
    • Dual specificity protein phosphatase PYST2 antibody
    • DUS7_HUMAN antibody
    • Dusp7 antibody
    • Jnk Stimulatory Phosphatase 1 antibody
    • JSP1 antibody
    • MAP kinase phosphatase X antibody
    • MKPX antibody
    • PYST2 antibody
    see all

Images

  • Anti-DUSP7 antibody (ab100921) at 1/500 dilution + HepG2 Cell Lysate

    Predicted band size: 41 kDa

  • ab100921, at 1/100 dilution, staining cytoplasmic DUSP7 in formalin-fixed, paraffin-embedded Human fetal liver tissue, by Immunohistochemistry.

References

This product has been referenced in:

  • Jafarnejad SM  et al. Translational control of ERK signaling through miRNA/4EHP-directed silencing. Elife 7:N/A (2018). Read more (PubMed: 29412140) »
  • Carpio LR  et al. Histone deacetylase 3 suppresses Erk phosphorylation and matrix metalloproteinase (Mmp)-13 activity in chondrocytes. Connect Tissue Res 58:27-36 (2017). Read more (PubMed: 27662443) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary MEF)
Loading amount
5 µg
Specification
Primary MEF
Treatment
100ngµl TPA for 2 hours
Gel Running Conditions
Reduced Denaturing (4-14% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 28 2012

Answer

I just wanted to check in with you regarding the issues that you had been experiencing the DUSP7 and DUSP2 antibodies.

Have you had an opportunity to assemble information about your protocol? We take issues such as these seriously and we guarantee our products for a period of 6 months if used the manner specified on our datasheets. We ask for protocol details to determine if the product is being used consistent with the Abpromise.

It is also very important that we have the Abcam confirmation number or the PO number used to purchase the product as I would need these to issue a replacement or refund.

Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Answer

Thank you for your response.

I am sorry about the issues you have been having. Thank you for bringing this to our attention here and with Jason. Unfortunately the image did not arrive with this. It may be that the file size is too large for our email system.

As company policy, in order to send a replacement we do need the particulars of your protocol. This information is important not only in helping us understand the problems that you are experiencing but in our internal testing as well.

I see that you have been working with Mouse ESCs, could you tell me the lysis buffer that you have used?
Have you reduced and denatured your samples? Which method/buffers did you use?
How much total protein did you use per well?
Did you load a loading control? Which lc did you use? What were the results?
Did you load a positive control? Which pc did you use? What were the results?
What membrane type did you transfer to?
What blocking steps did you use?
What dilutions of this product did you use? How long and at what temperature was this antibody incubated?
What was the secondary antibody that you have used?How long and at what temperature was this antibody incubated?
Have you run a no primary antibody control blot? What were the results?
What is the wash buffer that you use?
Could you provide the Abcam order confirmation number or PO number used to purchase this?

Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.

Read More

Answer

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Often times high background can be reduced by using TBS + 0.1% Tween 20 for your washes or by reducing the concentration of your antibodies. Would you mind sending me the details of your protocol including the tissue type you are working with, your sample preparations including denaturing and reducing steps, the amount of total protein per well, the positive controls you have used, loading controls or poncheau staining results, blocking procedures, antibody incubation steps, washes, images and any steps that you may have changed while troubleshooting.

These details will not only help me to better understand your difficulties and be able to offer suggestions but will also be used for internal testing of the products if deemed neccessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee.


Please let me know if you have any questions.

Read More

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