Overview

  • Product name

  • Description

    Goat polyclonal to DUSP8
  • Host species

    Goat
  • Specificity

    This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AGDRLPRKVMDAK, corresponding to N terminal amino acids 2-14 of Human DUSP8.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml.

Approx. 60-65kDa bands seen in Human Heart lysate.

Target

  • Function

    This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.
  • Tissue specificity

    Abundant in brain, heart and skeletal muscle.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C11orf81 antibody
    • Chromosome 11 open reading frame 81 antibody
    • Deubiquitinating enzyme 8 antibody
    • Dual Specificity Phosphatase 8 antibody
    • Dual specificity phosphatase M3/6 antibody
    • Dual specificity protein phosphatase 8 antibody
    • Dual specificity protein phosphatase hVH 5 antibody
    • Dual specificity protein phosphatase hVH-5 antibody
    • DUS8 antibody
    • DUS8_HUMAN antibody
    • DUSP8 antibody
    • EC 3.1.3.16 antibody
    • EC 3.1.3.48 antibody
    • FLJ42476 antibody
    • FLJ42958 antibody
    • H1 phosphatase vaccinia virus homolog antibody
    • H1 phosphatase, vaccinia virus, homolog of antibody
    • HB5 antibody
    • hUBPy antibody
    • HVH5 antibody
    • HVH8 antibody
    • M3/6, mouse, homolog of antibody
    • Neuronal tyrosine threonine phosphatase 1 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Ubiquitin carboxyl terminal hydrolase 8 antibody
    • Ubiquitin specific processing protease 8 antibody
    • Ubiquitin thiolesterase 8 antibody
    • VH5 antibody
    see all

Images

  • Ab1363 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Ab1363 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

References

ab1363 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. I am sorry to hear you are having a problem with ab1363. I would like to suggest the following modifications to your protocol: One change I can suggest in your protocol is to do 5 x 5 minutes washes for primary and secondary antibodies as opposed to 3 x 10 minutes. More frequent but shorter washes will get rid of unbound antibody better. To clarify your question at the end of the questionnaire, this antibody is specific to DUSP, not USP. Also, we recommend using BSA in the blocking step as opposed to milk. If these modifications do not help you to get better results, please let me know and we can discuss a free of charge replacement or a refund. Please let me know if this helps and do not hesitate to contact us for further advice.

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