Anti-DUSP9 antibody - C-terminal (ab152119)
Key features and details
- Rabbit polyclonal to DUSP9 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DUSP9 antibody - C-terminal
See all DUSP9 primary antibodies -
Description
Rabbit polyclonal to DUSP9 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 269-360 of Human DUSP9 (NP_001386).
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Positive control
- A431 whole cell lysate; A431 cells; NCIN87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Inactivates MAP kinases. Has a specificity for the ERK family. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 614306 Cow
- Entrez Gene: 1852 Human
- Entrez Gene: 75590 Mouse
- Entrez Gene: 293847 Rat
- Omim: 300134 Human
- SwissProt: Q99956 Human
- Unigene: 144879 Human
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Alternative names
- Dual specificity phosphatase 9 antibody
- Dual specificity protein phosphatase 9 antibody
- DUS9_HUMAN antibody
see all
Images
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Anti-DUSP9 antibody - C-terminal (ab152119) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 42 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde fixed A431 cells, labeling DUSP9 with ab152119 at 1/200 dilution. Lower image merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue, labeling DUSP9 with ab152119 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab152119 has been referenced in 1 publication.
- Aurtenetxe O et al. DUSP5 expression associates with poor prognosis in human neuroblastoma. Exp Mol Pathol 105:272-278 (2018). PubMed: 30171833