Overview

  • Product name

    Anti-DUSP9 antibody [EPR15541]
    See all DUSP9 primary antibodies
  • Description

    Rabbit monoclonal [EPR15541] to DUSP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DUSP9 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99956

  • Positive control

    • HeLa and 293 cell lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15541
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/100.

Target

  • Function

    Inactivates MAP kinases. Has a specificity for the ERK family.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity phosphatase 9 antibody
    • Dual specificity protein phosphatase 9 antibody
    • DUS9_HUMAN antibody
    • DUSP9 antibody
    • MAP kinase phosphatase 4 antibody
    • Mitogen activated protein kinase phosphatase 4 antibody
    • Mitogen-activated protein kinase phosphatase 4 antibody
    • MKP 4 antibody
    • MKP-4 antibody
    • MKP4 antibody
    • serine/threonine specific protein phosphatase antibody
    see all

Images

  • All lanes : Anti-DUSP9 antibody [EPR15541] (ab194355) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling DUSP9 with ab194355 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

References

ab194355 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194355.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up