Recombinant Anti-dUTPase antibody [EPR9608(B)] (ab137097)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9608(B)] to dUTPase
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-dUTPase antibody [EPR9608(B)]
See all dUTPase primary antibodies -
Description
Rabbit monoclonal [EPR9608(B)] to dUTPase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human dUTPase aa 200-300. The exact sequence is proprietary.
-
Positive control
- WB: HeLa, 293T, Ramos and HepG2 cell lysates; ICC: HepG2 cells; Flow Cyt (intra): SW620 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9608(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/250 - 1/500.
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.
|
|
Flow Cyt (Intra) |
1/1000.
|
Notes |
---|
ICC/IF
1/250 - 1/500. |
WB
1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa. |
Flow Cyt (Intra)
1/1000. |
Target
-
Function
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. -
Tissue specificity
Found in a variety of tissues. -
Pathway
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. -
Sequence similarities
Belongs to the dUTPase family. -
Post-translational
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1854 Human
- Omim: 601266 Human
- SwissProt: P33316 Human
- Unigene: 527980 Human
-
Alternative names
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
see all
Images
-
All lanes : Anti-dUTPase antibody [EPR9608(B)] (ab137097) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 26, 18 kDa -
Flow Cytometry analysis of SW620 (Human colorectal adenocarcinoma epithelial cell) cells labeling dUTPase with purified ab137097 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling dUTPase with ab137097 at 1/250 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab137097 has been referenced in 2 publications.
- Hewitt G et al. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Mol Cell 81:767-783.e11 (2021). PubMed: 33333017
- Chan M et al. Synergy between the NAMPT inhibitor GMX1777(8) and pemetrexed in non-small cell lung cancer cells is mediated by PARP activation and enhanced NAD consumption. Cancer Res 74:5948-54 (2014). PubMed: 25145669