Key features and details
- Rabbit polyclonal to dUTPase
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-dUTPase antibody
See all dUTPase primary antibodies
DescriptionRabbit polyclonal to dUTPase
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human dUTPase (internal sequence). The exact sequence is proprietary.
Database link: P33316
- WB: A431 whole cell lysate. IP: A431 whole cell extract. IHC-P: A549 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000.|
|IP||1/100 - 1/500.|
FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Tissue specificityFound in a variety of tissues.
PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similaritiesBelongs to the dUTPase family.
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
Anti-dUTPase antibody (ab229122) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
15% SDS-PAGE gel.
dUTPase was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 μg ab229122. Western blot was performed from the immunoprecipitate using ab229122. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in A431 whole cell extract.
Lane 2: ab229122 IP in A431 whole cell extract.
Paraffin-embedded A549 xenograft tissue stained for dUTPase using ab229122 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229122 has not yet been referenced specifically in any publications.