Overview

  • Product name

  • Description

    Rabbit polyclonal to dUTPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human dUTPase (internal sequence). The exact sequence is proprietary.
    Database link: P33316

  • Positive control

    • WB: A431 whole cell lysate. IP: A431 whole cell extract. IHC-P: A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab229122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000.
IP 1/100 - 1/500.

Target

  • Function

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • Tissue specificity

    Found in a variety of tissues.
  • Pathway

    Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
  • Sequence similarities

    Belongs to the dUTPase family.
  • Post-translational
    modifications

    Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
  • Cellular localization

    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
    • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
    • Deoxyuridine triphosphatase antibody
    • dut antibody
    • DUT_HUMAN antibody
    • dUTP nucleotidohydrolase antibody
    • dUTP pyrophosphatase antibody
    • dUTPase antibody
    • FLJ20622 antibody
    see all

Images

  • Anti-dUTPase antibody (ab229122) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg


    15% SDS-PAGE gel.

  • dUTPase was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 μg ab229122. Western blot was performed from the immunoprecipitate using ab229122. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in A431 whole cell extract.
    Lane 2: ab229122 IP in A431 whole cell extract.

  • Paraffin-embedded A549 xenograft tissue stained for dUTPase using ab229122 at 1/500 dilution in immunohistochemical analysis.

References

ab229122 has not yet been referenced specifically in any publications.

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