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RecombinantRabMAb

Recombinant Anti-dUTPase antibody [EPR9607(B)] (ab137102)

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  • SDS
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Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
  • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
  • Anti-dUTPase antibody [EPR9607(B)] (ab137102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9607(B)] to dUTPase
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-dUTPase antibody [EPR9607(B)]
    See all dUTPase primary antibodies
  • Description

    Rabbit monoclonal [EPR9607(B)] to dUTPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9607(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Associated products

  • Alternative Versions

    • Anti-dUTPase antibody [EPR9607(B)] - BSA and Azide free (ab248819)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250 - 1/500.
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
Notes
ICC/IF
1/250 - 1/500.
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificity

      Found in a variety of tissues.
    • Pathway

      Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similarities

      Belongs to the dUTPase family.
    • Post-translational
      modifications

      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localization

      Mitochondrion and Nucleus.
    • Target information above from: UniProt accession P33316 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1854 Human
      • Omim: 601266 Human
      • SwissProt: P33316 Human
      • Unigene: 527980 Human
      • Alternative names

        • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
        • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
        • Deoxyuridine triphosphatase antibody
        • dut antibody
        • DUT_HUMAN antibody
        • dUTP nucleotidohydrolase antibody
        • dUTP pyrophosphatase antibody
        • dUTPase antibody
        • FLJ20622 antibody
        see all

      Images

      • Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 26 kDa
        Observed band size: 18 kDa why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)

        Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.

      • Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Anti-dUTPase antibody [EPR9607(B)] (ab137102)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab137102? Please let us know so that we can cite the reference in this datasheet.

      ab137102 has not yet been referenced specifically in any publications.

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