Recombinant
RabMAb

Recombinant Anti-dUTPase antibody [EPR9607(B)] (ab137102)

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Overview

  • Product name

    Anti-dUTPase antibody [EPR9607(B)]
    See all dUTPase primary antibodies

  • Description

    Rabbit monoclonal [EPR9607(B)] to dUTPase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

    • Tissue specificity

      Found in a variety of tissues.

    • Pathway

      Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

    • Sequence similarities

      Belongs to the dUTPase family.

    • Post-translational
      modifications

      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.

    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
        • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
        • Deoxyuridine triphosphatase antibody
        • dut antibody
        • DUT_HUMAN antibody
        • dUTP nucleotidohydrolase antibody
        • dUTP pyrophosphatase antibody
        • dUTPase antibody
        • FLJ20622 antibody
        see all

      Images

      • Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 26 kDa
        Observed band size: 18 kDa
        why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)

        Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.

      References

      ab137102 has not yet been referenced specifically in any publications.

      Publishing research using ab137102? Please let us know so that we can cite the reference in this datasheet.

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