Recombinant Anti-dUTPase antibody [EPR9607(B)] (ab137102)
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- References
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Overview
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Product name
Anti-dUTPase antibody [EPR9607(B)]
See all dUTPase primary antibodies -
Description
Rabbit monoclonal [EPR9607(B)] to dUTPase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9607(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250 - 1/500. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. -
Tissue specificity
Found in a variety of tissues. -
Pathway
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. -
Sequence similarities
Belongs to the dUTPase family. -
Post-translational
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1854 Human
- Omim: 601266 Human
- SwissProt: P33316 Human
- Unigene: 527980 Human
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Alternative names
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
see all
Images
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![Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)](https://www.abcam.com/ps/products/137/ab137102/Images/DUT-Primary-antibodies-ab137102-1.JPG)
Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)](https://www.abcam.com/ps/products/137/ab137102/Images/DUT-Primary-antibodies-ab137102-2.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.
Datasheets and documents
References
ab137102 has not yet been referenced specifically in any publications.
