RecombinantRabMAb

Recombinant Anti-dUTPase antibody [EPR9607(B)] (ab137102)

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Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
  • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
  • Anti-dUTPase antibody [EPR9607(B)] (ab137102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9607(B)] to dUTPase
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-dUTPase antibody [EPR9607(B)]
    See all dUTPase primary antibodies

  • Description

    Rabbit monoclonal [EPR9607(B)] to dUTPase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250 - 1/500.
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
Notes
ICC/IF
1/250 - 1/500.
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

    • Tissue specificity

      Found in a variety of tissues.

    • Pathway

      Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

    • Sequence similarities

      Belongs to the dUTPase family.

    • Post-translational
      modifications

      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.

    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
        • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
        • Deoxyuridine triphosphatase antibody
        • dut antibody
        • DUT_HUMAN antibody
        • dUTP nucleotidohydrolase antibody
        • dUTP pyrophosphatase antibody
        • dUTPase antibody
        • FLJ20622 antibody
        see all

      Images

      • Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Western blot - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 26 kDa
        Observed band size: 18 kDa why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9607(B)] (ab137102)

        Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.

      • Anti-dUTPase antibody [EPR9607(B)] (ab137102)
        Anti-dUTPase antibody [EPR9607(B)] (ab137102)

      References (0)

      Publishing research using ab137102? Please let us know so that we can cite the reference in this datasheet.

      ab137102 has not yet been referenced specifically in any publications.

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