Overview

  • Product name

    Anti-dUTPase antibody [EPR9607(B)]
    See all dUTPase primary antibodies
  • Description

    Rabbit monoclonal [EPR9607(B)] to dUTPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificity

      Found in a variety of tissues.
    • Pathway

      Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similarities

      Belongs to the dUTPase family.
    • Post-translational
      modifications

      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Images

    • All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.

    References

    ab137102 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137102.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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