Product nameAnti-dUTPase antibody [EPR9607(B)]
See all dUTPase primary antibodies
DescriptionRabbit monoclonal [EPR9607(B)] to dUTPase
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human dUTPase aa 50-150. The exact sequence is proprietary.
- HeLa, 293T, Jurkat and HepG2 cell lysates; HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250 - 1/500.|
|WB||1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 26 kDa).|
FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Tissue specificityFound in a variety of tissues.
PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similaritiesBelongs to the dUTPase family.
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
All lanes : Anti-dUTPase antibody [EPR9607(B)] (ab137102) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of HeLa cells labelling dUTPase with ab137102 at 1/250 dilution.
ab137102 has not yet been referenced specifically in any publications.