Product nameAnti-dUTPase antibody [EPR9608(B)]
See all dUTPase primary antibodies
DescriptionRabbit monoclonal [EPR9608(B)] to dUTPase
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human dUTPase aa 200-300. The exact sequence is proprietary.
- HeLa, 293T, Ramos and HepG2 cell lysates; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250 - 1/500.|
|IP||1/100 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.|
FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Tissue specificityFound in a variety of tissues.
PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similaritiesBelongs to the dUTPase family.
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
All lanes : Anti-dUTPase antibody [EPR9608(B)] (ab137097) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 26, 18 kDa
Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling dUTPase with ab137097 at 1/250 dilution.
This product has been referenced in:
- Chan M et al. Synergy between the NAMPT inhibitor GMX1777(8) and pemetrexed in non-small cell lung cancer cells is mediated by PARP activation and enhanced NAD consumption. Cancer Res 74:5948-54 (2014). Read more (PubMed: 25145669) »