Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9608(B)] to dUTPase
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-dUTPase antibody [EPR9608(B)]
See all dUTPase primary antibodies
DescriptionRabbit monoclonal [EPR9608(B)] to dUTPase
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human dUTPase aa 200-300. The exact sequence is proprietary.
- HeLa, 293T, Ramos and HepG2 cell lysates; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250 - 1/500.|
|IP||1/100 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26, 18 kDa.|
FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Tissue specificityFound in a variety of tissues.
PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similaritiesBelongs to the dUTPase family.
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
All lanes : Anti-dUTPase antibody [EPR9608(B)] (ab137097) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 26, 18 kDa
Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling dUTPase with ab137097 at 1/250 dilution.
ab137097 has been referenced in 1 publication.
- Chan M et al. Synergy between the NAMPT inhibitor GMX1777(8) and pemetrexed in non-small cell lung cancer cells is mediated by PARP activation and enhanced NAD consumption. Cancer Res 74:5948-54 (2014). PubMed: 25145669