Recombinant Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)
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- References
- Protocols
Overview
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Product name
Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free
See all dUTPase primary antibodies -
Description
Rabbit monoclonal [EPR9608(B)] to dUTPase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human dUTPase aa 200-300. The exact sequence is proprietary.
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Positive control
- ICC/IF: HepG2 cells.
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General notes
ab232044 is the carrier-free version of ab137097 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232044 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9608(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab232044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. -
Tissue specificity
Found in a variety of tissues. -
Pathway
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. -
Sequence similarities
Belongs to the dUTPase family. -
Post-translational
modificationsPhosphorylation in mature T-cells occur in a cell cycle-dependent manner. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1854 Human
- Omim: 601266 Human
- SwissProt: P33316 Human
- Unigene: 527980 Human
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Alternative names
- Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
- Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
- Deoxyuridine triphosphatase antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)](https://www.abcam.com/ps/products/232/ab232044/Images/ab232044-308888-044.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)
Datasheets and documents
References
ab232044 has not yet been referenced specifically in any publications.
