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RecombinantRabMAb

Recombinant Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)
  • Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9608(B)] to dUTPase - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free
    See all dUTPase primary antibodies
  • Description

    Rabbit monoclonal [EPR9608(B)] to dUTPase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human dUTPase aa 200-300. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HepG2 cells.
  • General notes

    ab232044 is the carrier-free version of ab137097 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232044 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9608(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Associated products

  • Alternative Versions

    • Anti-dUTPase antibody [EPR9608(B)] (ab137097)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab232044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.

Target

  • Function

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • Tissue specificity

    Found in a variety of tissues.
  • Pathway

    Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
  • Sequence similarities

    Belongs to the dUTPase family.
  • Post-translational
    modifications

    Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
  • Cellular localization

    Mitochondrion and Nucleus.
  • Target information above from: UniProt accession P33316 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1854 Human
    • Omim: 601266 Human
    • SwissProt: P33316 Human
    • Unigene: 527980 Human
    • Alternative names

      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)
      Immunocytochemistry/ Immunofluorescence - Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)

      Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling dUTPase with ab137097 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137097).

    • Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)
      Anti-dUTPase antibody [EPR9608(B)] - BSA and Azide free (ab232044)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab232044? Please let us know so that we can cite the reference in this datasheet.

    ab232044 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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