Overview

  • Product name

    Anti-Dux4 antibody [E5-5]
    See all Dux4 primary antibodies
  • Description

    Rabbit monoclonal [E5-5] to Dux4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    .

  • Positive control

    • WB: Human testis tissue lysates. HT-1080, PC-3 Saos-2 and HeLa whole cell lysate (ab150035).
  • General notes

    For details on the development of this clone, please refer to Geng et al.  Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E5-5
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Relevance

      DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • Double homeobox 4 antibody
      • Double homeobox protein 10 antibody
      • Double homeobox protein 4 antibody
      • Double homeobox protein 4/10 antibody
      • DUX10 antibody
      • DUX4L antibody
      • DUX4L1 antibody
      see all

    Images

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : HT1080 (human fibrosarcoma cell line) whole cell lysate
      Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 3 : Saos-2 (human osteosarcoma cell line) whole cell lysate
      Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : HT1080 (human fibrosarcoma cell line) cell lysate
      Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
      Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 45 kDa

    References

    This product has been referenced in:

    • Mitsuhashi H  et al. Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers. Sci Rep 9:2274 (2019). Read more (PubMed: 30783185) »
    • Jones T & Jones PL A cre-inducible DUX4 transgenic mouse model for investigating facioscapulohumeral muscular dystrophy. PLoS One 13:e0192657 (2018). IHC-P ; Mouse . Read more (PubMed: 29415061) »
    See all 14 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse E14 ES cells)
    Permeabilization
    Yes - Triton
    Specification
    Mouse E14 ES cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Dr. Diego Rodriguez Terrones

    Verified customer

    Submitted Mar 28 2019

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse embryonic and induced pluripotent stem cells)
    Permeabilization
    Yes - tritons-100
    Specification
    mouse embryonic and induced pluripotent stem cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.004% · Temperature: 4°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 15 2018

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Rhabdomyosarcoma cell line)
    Specification
    Rhabdomyosarcoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up