DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)

Submit a review Submit a question References (2)

Key features and details

  • DyLight® 488 Mouse monoclonal [AD1.1.10] to 6X His tag®
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Species independent
  • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
  • Isotype: IgG1

Overview

  • Product name

    DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]
    See all 6X His tag® primary antibodies

  • Description

    DyLight® 488 Mouse monoclonal [AD1.1.10] to 6X His tag®

  • Host species

    Mouse

  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide of 6X His tag®.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, 0.88% Sodium chloride, 80.5% Dibasic monohydrogen sodium phosphate, 18.5% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    AD1.1.10

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References (2)

Publishing research using ab117512? Please let us know so that we can cite the reference in this datasheet.

ab117512 has been referenced in 2 publications.

  • Ding Y  et al. Optimized Anti-Prostate-Specific Membrane Antigen Single-Chain Variable Fragment-Loaded Nanobubbles as a Novel Targeted Ultrasound Contrast Agent for the Diagnosis of Prostate Cancer. J Ultrasound Med 39:761-773 (2020). PubMed: 31702068
  • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab117512.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com