DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
Key features and details
- DyLight® 488 Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
DyLight® 488 Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
Sequence:DYKDDDDK
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
M2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
- SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
- Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/5000. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab117505 has been referenced in 2 publications.
- Yoon S et al. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron 105:506-521.e7 (2020). PubMed: 31813652
- Yoon S et al. TGF-ß-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance. Cell Rep 31:107685 (2020). PubMed: 32460012
