DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)
![Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)](https://www.abcam.com/ps/products/117/ab117488/Images/ab117488-213661-ab117488icc.jpg "Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)")
Key features and details
- DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin
- Suitable for: ICC/IF, Flow Cyt
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Isotype: IgG1
Overview
-
Product name
DyLight® 488 Anti-Hemagglutinin antibody [16B12]
See all Hemagglutinin primary antibodies -
Description
DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin -
Host species
Mouse -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Immunogen
The influenza hemagglutinin epitope (YPYDVPDYA).
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.6% Sodium phosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab117488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/5000. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
-
Relevance
Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. -
Cellular localization
Cell membrane; apical cell membrane; single-pass type I membrane protein. -
Alternative names
- HA antibody
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
Images
-
Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)This image is courtesy of an Abreview submitted by Ya Zhou
ab117488 staining Hemagglutinin in Monkey cos7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C.
Protocols
References (1)
ab117488 has been referenced in 1 publication.
- Zhang Q et al. Site-specific polyubiquitination differentially regulates parathyroid hormone receptor-initiated MAPK signaling and cell proliferation. J Biol Chem 293:5556-5571 (2018). PubMed: 29444827
