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  1. Link

    dylight-488-hemagglutinin-antibody-16b12-ab117488.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Influenza
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DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)

    Key features and details

    • DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin
    • Suitable for: ICC/IF, Flow Cyt
    • Reacts with: Influenza A
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Isotype: IgG1

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    Overview

    • Product name

      DyLight® 488 Anti-Hemagglutinin antibody [16B12]
      See all Hemagglutinin primary antibodies
    • Description

      DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin
    • Host species

      Mouse
    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm
    • Tested applications

      Suitable for: ICC/IF, Flow Cytmore details
    • Species reactivity

      Reacts with: Influenza A
    • Immunogen

      The influenza hemagglutinin epitope (YPYDVPDYA).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 1.6% Sodium phosphate, 0.88% Sodium chloride, 1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      16B12
    • Isotype

      IgG1
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Influenza

    Associated products

    • Recombinant Protein

      • Recombinant Influenza A Hemagglutinin protein (His tag) Strain Perth/16/09 (ab217652)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab117488 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF (1)
    1/500 - 1/5000.
    Flow Cyt
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    1/500 - 1/5000.
    Flow Cyt
    Use at an assay dependent concentration.

    Target

    • Relevance

      Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
    • Cellular localization

      Cell membrane; apical cell membrane; single-pass type I membrane protein.
    • Database links

      • Entrez Gene: 3654620 Influenza A
      • SwissProt: C3W5S1 Influenza A
      • SwissProt: Q0HD60 Influenza A
      • SwissProt: Q9Q0U6 Influenza A
      • Alternative names

        • HA antibody
        • HA1 antibody
        • HA2 antibody
        • Hemagglutinin antibody

      Images

      • Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)
        Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)This image is courtesy of an Abreview submitted by Ya Zhou

        ab117488 staining Hemagglutinin in Monkey cos7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C.

        See Abreview

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab117488? Please let us know so that we can cite the reference in this datasheet.

      ab117488 has been referenced in 1 publication.

      • Zhang Q  et al. Site-specific polyubiquitination differentially regulates parathyroid hormone receptor-initiated MAPK signaling and cell proliferation. J Biol Chem 293:5556-5571 (2018). PubMed: 29444827

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Hemagglutinin antibody [16B12] (DyLight® 488)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Monkey Cell (cos7)
      Specification
      cos7
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Ya Zhou

      Verified customer

      Submitted Dec 13 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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