DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)
Key features and details
- DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Influenza A
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Isotype: IgG1
Overview
-
Product name
DyLight® 488 Anti-Hemagglutinin antibody [16B12]
See all Hemagglutinin primary antibodies -
Description
DyLight® 488 Mouse monoclonal [16B12] to Hemagglutinin -
Host species
Mouse -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
The influenza hemagglutinin epitope (YPYDVPDYA).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.6% Sodium phosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/500 - 1/5000.
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
ICC/IF
1/500 - 1/5000. |
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Relevance
Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. -
Cellular localization
Cell membrane; apical cell membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 3654620 Influenza A
- SwissProt: C3W5S1 Influenza A
- SwissProt: Q0HD60 Influenza A
- SwissProt: Q9Q0U6 Influenza A
-
Alternative names
- HA antibody
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
Images
-
Immunocytochemistry/ Immunofluorescence - DyLight® 488 Anti-Hemagglutinin antibody [16B12] (ab117488)This image is courtesy of an Abreview submitted by Ya Zhou
ab117488 staining Hemagglutinin in Monkey cos7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab117488 has been referenced in 1 publication.
- Zhang Q et al. Site-specific polyubiquitination differentially regulates parathyroid hormone receptor-initiated MAPK signaling and cell proliferation. J Biol Chem 293:5556-5571 (2018). PubMed: 29444827