DyLight® 488 Anti-KDEL antibody [10C3] - ER Marker (ab115638)

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Flow Cytometry - DyLight® 488 Anti-KDEL antibody [10C3] - ER Marker (ab115638)

    Key features and details

    • DyLight® 488 Mouse monoclonal [10C3] to KDEL - ER Marker
    • Suitable for: Flow Cyt
    • Reacts with: Rat, Human
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Isotype: IgG2a

    Overview

    • Product name

      DyLight® 488 Anti-KDEL antibody [10C3] - ER Marker
      See all KDEL primary antibodies

    • Description

      DyLight® 488 Mouse monoclonal [10C3] to KDEL - ER Marker

    • Host species

      Mouse

    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm

    • Specificity

      Recognizes proteins containing the KDEL sequence.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Rat, Human
      Predicted to work with: Bird, Plants, Mammals

    • Immunogen

      Synthetic peptide corresponding to Rat KDEL aa 649-654.
      Sequence:

      SEKDEL

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Jurkat cells.

    • General notes

      This product was changed from ascites to tissue culture supernatant on 12th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      Purified from TCS

    • Clonality

      Monoclonal

    • Clone number

      10C3

    • Isotype

      IgG2a

    • Research areas

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab115638 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/20.

    Target

    • Relevance

      The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

    • Cellular localization

      Endoplasmic reticulum

    • Alternative names

      • KDEL antibody
      • Lys Asp Glu Leu antibody

    Images

    • Flow Cytometry - DyLight® 488 Anti-KDEL antibody [10C3] - ER Marker (ab115638)
      Flow Cytometry - DyLight® 488 Anti-KDEL antibody [10C3] - ER Marker (ab115638)

      ab115638 at 10µg/ml staining KDEL in 106 Jurkat cells by Flow cytometry (shown in green).
      Grey: unstained;Yellow: isotype control.

    References (1)

    Publishing research using ab115638? Please let us know so that we can cite the reference in this datasheet.

    ab115638 has been referenced in 1 publication.

    • Lee JH  et al. Hypoxic Preconditioning Promotes the Bioactivities of Mesenchymal Stem Cells via the HIF-1a-GRP78-Akt Axis. Int J Mol Sci 18:N/A (2017). Flow Cyt ; Human . PubMed: 28635661

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    Hello. I want to use this antibody for FACS to sort cells and do not want to permeabilize. Have you tested the antibody without permeailization? Thank you!

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    Answer

    Thank you for contacting us. This antibody has not been tested without permeabilization. Due to its intracellular localization, the laboratory skeptical that it would work this way.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Cusotmer kindly contacted us regarding the protocol for use of this product in flow cytometry.

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    Answer

    Thank you for contacting us regarding the flow cytometry protocol for ab115638.

    I do wish to apologize for the delay in getting this information to you. The protocol used by the lab is attached.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Question

    Dear Madam/Sir,



    My name is Junhua Mai, who is a research fellow in the Methodist Hospital Research Institute, Houston.

    For the product “Anti-GRP78 antibody [10C3] (DyLight® 488) (ab115638)”, did you provide relative isotype controls? I cannot find the IgG2a-dylight 488 isotype control in the online catalog.



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    Answer

    Thank you for contacting us. Unofrtunately we do not have the Dylight 488 isotype control available in our catalog.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    The datasheet for this antibody indicates that only rat is tested, however the image is of data from Jurkat cells. Is human a tested species guaranteed by the Abpromise?

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    Answer

    Thank you for contacting Abcam and for your interest in ab115638.


    As we discussed, I have updated the datasheet to reflect that human is a tested species guaranteed by our Abpromise. I apologize for the discrepancy and for any confusion this may have caused. Thank you for pointing out this error.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions. I wish you the best of luck with your experiments!

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com