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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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DyLight® 488 Anti-Ubiquitin antibody (ab115637)

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Flow Cytometry - DyLight® 488 Anti-Ubiquitin antibody (ab115637)

    Key features and details

    • DyLight® 488 Rabbit polyclonal to Ubiquitin
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Fish, Monkey, Xenopus tropicalis
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Ubiquitin protein (His tag) (ab218616)

    View more associated products

    Overview

    • Product name

      DyLight® 488 Anti-Ubiquitin antibody
      See all Ubiquitin primary antibodies
    • Description

      DyLight® 488 Rabbit polyclonal to Ubiquitin
    • Host species

      Rabbit
    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Fish, Monkey, Xenopus tropicalis
    • Immunogen

      Native bovine Ubiquitin protein.

    • Positive control

      • Jurkat cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Ubiquitylation
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other

    Associated products

    • Isotype control

      • DyLight® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab153686)
    • Recombinant Protein

      • Recombinant Human Ubiquitin protein (His tag) (ab218616)

    Applications

    Our Abpromise guarantee covers the use of ab115637 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/20.

    Target

    • Relevance

      Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localization

      Cell Membrane, Cytoplasmic and Nuclear
    • Database links

      • Entrez Gene: 101747587 Chicken
      • Entrez Gene: 281370 Cow
      • Entrez Gene: 38456 Drosophila melanogaster
      • Entrez Gene: 7314 Human
      • Entrez Gene: 22187 Mouse
      • Entrez Gene: 100125968 Pig
      • Entrez Gene: 192255 Rat
      • SwissProt: P0CG62 Chicken
      • SwissProt: P0CG53 Cow
      • SwissProt: P0CG69 Drosophila melanogaster
      • SwissProt: P68198 Drosophila melanogaster
      • SwissProt: P0CG47 Human
      • SwissProt: P0CG48 Human
      • SwissProt: P62979 Human
      • SwissProt: P62987 Human
      • SwissProt: P62988 Human
      • SwissProt: P0CG49 Mouse
      • SwissProt: P62991 Mouse
      • SwissProt: P0CG51 Rat
      • SwissProt: P62989 Rat
      • Unigene: 2641 Drosophila melanogaster
      • Unigene: 356190 Human
      • Unigene: 520348 Human
      • Unigene: 282093 Mouse
      • Unigene: 371592 Mouse
      • Unigene: 110618 Rat
      • Unigene: 1253 Rat
      • Unigene: 3761 Rat
      see all
    • Alternative names

      • Epididymis secretory protein Li 50 antibody
      • FLJ25987 antibody
      • HEL S 50 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Images

    • Flow Cytometry - DyLight® 488 Anti-Ubiquitin antibody (ab115637)
      Flow Cytometry - DyLight® 488 Anti-Ubiquitin antibody (ab115637)
      ab115637 at 50µg/ml staining Ubiquitin in 106 Jurkat cells by Flow cytometry (shown in green).
      Grey: unstained;Yellow: isotype control.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab115637? Please let us know so that we can cite the reference in this datasheet.

    ab115637 has been referenced in 3 publications.

    • Garcia CS  et al. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells. An Acad Bras Cienc 88:281-92 (2016). PubMed: 26839997
    • de S Wisintainer GG  et al. O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines. Genet Mol Res 14:17472-81 (2015). PubMed: 26782390
    • Frozza CO  et al. Proteomic analysis identifies differentially expressed proteins after red propolis treatment in Hep-2 cells. Food Chem Toxicol 63:195-204 (2014). PubMed: 24239894

    Customer reviews and Q&As

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