DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)

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Key features and details

  • DyLight® 550 Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: ICC/IF
  • Reacts with: Species independent
  • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
  • Isotype: IgG1

Overview

  • Product name

    DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies

  • Description

    DyLight® 550 Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • Host species

    Mouse

  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1.6% Sodium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    M2

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab117495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/5000.
Notes
ICC/IF
1/500 - 1/5000.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).

  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

References (0)

Publishing research using ab117495? Please let us know so that we can cite the reference in this datasheet.

ab117495 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Submit a question

Question

I am interested by your AntiFLAG DyLight550 (ab117495).

Could you tell me approximateley how many assays in 200 uL samples can be made with 100 ug of AntiFLAG?

Could you propose us a discount as we are academic researchers from the EPFL?

Read More
Answer

Unfortunately, the volume of this antibody is batch dependent, and hence so will the working concentration. In addition, the working dilution will depend on the expression level of the tagged protein. Therefore, we cannot tell how many assays you will be able to perfom with this antibody.

Regarding the discount, please contact our customer service under orders@abcam.comto check if we could offer you a discount.

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