Overview

  • Product name
    DyLight® 550 Fast Conjugation Kit
  • Product overview

    The Abcam Dylight ® 550 Fast Conjugation Kit (ab201800) provides an easy-to-use, one-step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Dylight ® 550 with only 30 seconds hands-on time. Furthermore, conjugates are ready to use in less than twenty minutes.

    Dylight ® 550 is a orange fluorescent dye commonly used in fluorescence microscopy, arrays and in situ hybridiszation. It has an excitation wavelength of 562 nm and an emission wavelength of 576 nm (extinction coefficient 1.5 x 105 cm-1 M-1) and high quantum yield.

    Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.

     

     

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    30 μg containing 3 reactions (each for 10 - 20 μg)

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 30 µg 300 µg 1 mg
    Dylight® 550 Conjugation Mix 3 vials 3 vials 1 vial
    Dylight® 550 Modifier Reagent 1 vial 1 vial 1 vial
    Dylight® 550 Quencher Reagent 1 vial 1 vial 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

ab201800 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Improved staining for mouse-on-mouse immunofluorescence

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS.

Sections were then incubated with either:
(A) Mouse Anti-DDX4 (ab27591) at a 1:500 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Mouse 555 (ab150110) applied at a 1:500 dilution. (B) Mouse Anti-DDX4 (ab27591) labeled with Dylight 550 Fast Conjugation Kit (ab201800). Sections were incubated with labeled antibody at 1:100 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and mounted.

DDX4 is shown in red. Phalloidin, which stains F-actin is shown in blue.
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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