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  1. Link

    dylight-550-ha-tag-antibody-16b12-ab117502.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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DyLight® 550 Anti-HA tag antibody [16B12] (ab117502)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Key features and details

  • DyLight® 550 Mouse monoclonal [16B12] to HA tag
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Species independent
  • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
  • Isotype: IgG1

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Overview

  • Product name

    DyLight® 550 Anti-HA tag antibody [16B12]
    See all HA tag primary antibodies
  • Description

    DyLight® 550 Mouse monoclonal [16B12] to HA tag
  • Host species

    Mouse
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: 1.6% Sodium phosphate, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    16B12
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • Alternative Versions

    • DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)
    • PE Anti-HA tag antibody [16B12] (ab72479)
    • Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab117502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/5000.
Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/5000.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab117502? Please let us know so that we can cite the reference in this datasheet.

ab117502 has been referenced in 3 publications.

  • Castro-Gonzalez S  et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy 17:553-577 (2021). PubMed: 32097085
  • Morise J  et al. Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions. Int J Mol Sci 21:N/A (2020). PubMed: 32707739
  • Smith SE  et al. Interferon-induced Transmembrane Protein 1 restricts replication of virus that enter cells via the plasma membrane. J Virol N/A:N/A (2018). PubMed: 30567988

Customer reviews and Q&As

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