DyLight® 550 Anti-HA tag antibody [16B12] (ab117502)
Key features and details
- DyLight® 550 Mouse monoclonal [16B12] to HA tag
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Species independent
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Isotype: IgG1
Overview
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Product name
DyLight® 550 Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
DyLight® 550 Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.6% Sodium phosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab117502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | 1/500 - 1/5000. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Protocols
References (2)
ab117502 has been referenced in 2 publications.
- Morise J et al. Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions. Int J Mol Sci 21:N/A (2020). PubMed: 32707739
- Smith SE et al. Interferon-induced Transmembrane Protein 1 restricts replication of virus that enter cells via the plasma membrane. J Virol N/A:N/A (2018). PubMed: 30567988
