DyLight® 550 Anti-HA tag antibody [16B12] (ab117502)
Key features and details
- DyLight® 550 Mouse monoclonal [16B12] to HA tag
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Species independent
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Isotype: IgG1
Overview
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Product name
DyLight® 550 Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
DyLight® 550 Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to HA tag.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 1.6% Sodium phosphate, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
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| ICC/IF |
1/500 - 1/5000.
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| Notes |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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ICC/IF
1/500 - 1/5000. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab117502 has been referenced in 3 publications.
- Castro-Gonzalez S et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy 17:553-577 (2021). PubMed: 32097085
- Morise J et al. Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions. Int J Mol Sci 21:N/A (2020). PubMed: 32707739
- Smith SE et al. Interferon-induced Transmembrane Protein 1 restricts replication of virus that enter cells via the plasma membrane. J Virol N/A:N/A (2018). PubMed: 30567988