DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
![Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)](https://www.abcam.com/ps/products/117/ab117504/Images/ab117504-276742-anti-6x-his-tag-antibody-ad1110-dylight-650-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)")
Key features and details
- DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Overview
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Product name
DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm -
Specificity
ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresonding to 6X His tag®.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab133714)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- SureLight® P3 Anti-6X His tag® antibody [AD1.1.10] (ab72986)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/500 - 1/5000.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/5000. |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab117504 has been referenced in 5 publications.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Nguyen HQ et al. Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads. Elife 8:N/A (2019). PubMed: 31282865
- Harink B et al. An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs). PLoS One 14:e0203725 (2019). PubMed: 30901328
- Imhof BA et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114
- Tillotson BJ et al. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS One 10:e0145820 (2015). PubMed: 26713870
