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  1. Link

    dylight-650-6x-his-tag-antibody-ad1110-ab117504.pdf

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DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)

  • Datasheet
Reviews (2) Submit a question References (5)

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Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)

    Key features and details

    • DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Suitable for: ICC/IF, Flow Cyt
    • Reacts with: Species independent
    • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
    • Isotype: IgG1

    Overview

    • Product name

      DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
      See all 6X His tag® primary antibodies
    • Description

      DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Host species

      Mouse
    • Conjugation

      DyLight® 650. Ex: 654nm, Em: 673nm
    • Specificity

      ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.

    • Tested applications

      Suitable for: ICC/IF, Flow Cytmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide corresonding to 6X His tag®.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
    • Storage buffer

      pH: 8.20
      Constituent: 99% Tris buffered saline
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      AD1.1.10
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • His Tag

    Associated products

    • Alternative Versions

      • PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
      • DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
      • DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
      • SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab133714)
      • Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
      • Anti-6X His tag® antibody [AD1.1.10] (ab15149)
      • HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
      • Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
      • PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
      • SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
      • SureLight® P3 Anti-6X His tag® antibody [AD1.1.10] (ab72986)
      • Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)

    Applications

    Our Abpromise guarantee covers the use of ab117504 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/500 - 1/5000.
    Flow Cyt Use at an assay dependent concentration.

    Target

    • Relevance

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • Cellular localization

      Depends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
      Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)This image is courtesy of an anonymous abreview.

      Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab117504? Please let us know so that we can cite the reference in this datasheet.

    ab117504 has been referenced in 5 publications.

    • Del Toro D  et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
    • Nguyen HQ  et al. Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads. Elife 8:N/A (2019). PubMed: 31282865
    • Harink B  et al. An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs). PLoS One 14:e0203725 (2019). PubMed: 30901328
    • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114
    • Tillotson BJ  et al. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS One 10:e0145820 (2015). PubMed: 26713870

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Flow Cytometry abreview for Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (Yeast)
    Permeabilization
    No
    Gating Strategy
    No specific gating. Just the standard live cells and single cell population
    Specification
    Yeast
    Preparation
    Cell harvesting/tissue preparation method: No special procedure. Wash yeast cells with PBSA and stain with antibody 1:100 for 1 hr on ice. Wash with 1 ml PBSA and perform FACS
    Sample buffer: PBSA - PBS with 0.1% VSA
    Fixation
    No fixation
    Read More

    Dr. Kaushik Thakkar

    Verified customer

    Submitted Dec 18 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells transfected with His-tagged actin)
    Permeabilization
    Yes - 0.1% Triton-X100 in PBS
    Specification
    HeLa cells transfected with His-tagged actin
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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