DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
![Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)](https://www.abcam.com/ps/products/117/ab117504/Images/ab117504-276742-anti-6x-his-tag-antibody-ad1110-dylight-650-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)")
Key features and details
- DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Overview
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Product name
DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm -
Specificity
ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresonding to 6X His tag®.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab133714)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- SureLight® P3 Anti-6X His tag® antibody [AD1.1.10] (ab72986)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
Applications
Our Abpromise guarantee covers the use of ab117504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/5000. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.
Protocols
Datasheets and documents
References (5)
ab117504 has been referenced in 5 publications.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Nguyen HQ et al. Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads. Elife 8:N/A (2019). PubMed: 31282865
- Harink B et al. An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs). PLoS One 14:e0203725 (2019). PubMed: 30901328
- Imhof BA et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). PubMed: 27482114
- Tillotson BJ et al. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS One 10:e0145820 (2015). PubMed: 26713870
