DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)
![Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)](https://www.abcam.com/ps/products/117/ab117515/Images/ab117515-248151-anti-ha-tag-antibody-16b12-dylight-650-flow-cytometry.jpg "Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)")
Key features and details
- DyLight® 650 Mouse monoclonal [16B12] to HA tag
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Overview
-
Product name
DyLight® 650 Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
DyLight® 650 Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm -
Specificity
ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA). -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
-
General notes
Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab117515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/5000. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
-
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
-
Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)This image is courtesy of an anonymous Abreview
ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.
Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.
Grey - unstained cells.
Red - Wild type 293T, stained.
Blue - 293T expressing HA-tagged protein, stained.
Protocols
References (4)
ab117515 has been referenced in 4 publications.
- Lucken-Ardjomande Häsler S et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ?F508. J Cell Biol 219:N/A (2020). PubMed: 32344433
- Nafria M et al. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31:107691 (2020). PubMed: 32460028
- Feng Y et al. LRRC25 Functions as an Inhibitor of NF-?B Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Sci Rep 7:13448 (2017). PubMed: 29044191
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223
