DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)
![Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)](https://www.abcam.com/ps/products/117/ab117515/Images/ab117515-248151-anti-ha-tag-antibody-16b12-dylight-650-flow-cytometry.jpg "Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)")
Key features and details
- DyLight® 650 Mouse monoclonal [16B12] to HA tag
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Overview
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Product name
DyLight® 650 Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
DyLight® 650 Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm -
Specificity
ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA). -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/5000. |
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)This image is courtesy of an anonymous Abreview
ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.
Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.
Grey - unstained cells.
Red - Wild type 293T, stained.
Blue - 293T expressing HA-tagged protein, stained.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab117515 has been referenced in 6 publications.
- Lucken-Ardjomande Häsler S et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ?F508. J Cell Biol 219:N/A (2020). PubMed: 32344433
- Nafria M et al. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31:107691 (2020). PubMed: 32460028
- Gabaev I et al. Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation. Cell Rep 33:108249 (2020). PubMed: 33053346
- Marelli S et al. Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest. Elife 9:N/A (2020). PubMed: 32292164
- Feng Y et al. LRRC25 Functions as an Inhibitor of NF-?B Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Sci Rep 7:13448 (2017). PubMed: 29044191
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223